Determination of sulfonamide residues in animal foodstuffs by magnetic dispersive solid-phase extraction using magnetic carbon nanocomposites coupled with ion pair-dispersive liquid–liquid micro-extraction combined with HPLC-DAD

Abstract In this study, magnetic-dispersive solid-phase extraction coupled with ion pair-dispersive liquid–liquid micro-extraction (MSPE-i-DLLME) was used to the pre-concentration and of five sulfonamides residues (sulfadiazine, sulfathiazole, sulfacetamide, sulfamethazine sulfamethoxazole) in animal foodstuffs. The are extracted using magnetic carbon nanocomposite then eluted acetonitrile. DLLME step, target analytes collected 1-octanol containing 10% Aliquat-336 (as solvent). Finally, compounds quantified by HPLC DAD detection. parameters optimized one at time central composite design methods. Under conditions: sample solution volume 100 mL; initial pH: 12, amounts MCNs: 30 mg; desorption solvent (ACN) volume, 1 condition, 10 min sonication two step; (1-octanol + aliquat) 115 µL; pH 3; salt effect, 24.6%, (w/v) NaCl; centrifuge, 5 min, 4000 rpm. these conditions, proposed procedure represented good factors between 130 490; detection limits range from 0.01 μg kg −1 (at S / N = 3), linear response 0.1–400 concentration range. method is successfully applied determination